183 papers
Molecular biology explores the tiny machinery inside living cells, revealing how genetic instructions are read, copied, and turned into the proteins that keep us alive. This field acts as a bridge between the static code of DNA and the dynamic processes that drive growth, disease, and life itself, offering insights into everything from cellular repair to the development of new medicines.
On Gist.Science, we track every new preprint uploaded to bioRxiv in this category to make these complex discoveries accessible to everyone. Our team processes each submission to provide both clear, plain-language explanations and detailed technical summaries, ensuring you can grasp the core findings without getting lost in dense academic jargon.
Below are the latest molecular biology papers freshly processed from bioRxiv, ready for you to explore and understand.
This paper introduces an open framework for fine-tuning Boltz-2 using project-specific experimental data to significantly enhance its protein-ligand binding affinity prediction capabilities for lead optimization, achieving performance competitive with free energy perturbation methods.
This study demonstrates that an in vitro-reconstituted Drosophila Arc1 capsid, engineered to bind the mammalian receptor SORCS2, efficiently delivers Cas9 gene editors to dystrophic muscle in mdx mice, achieving significant exon skipping and restoration of dystrophin expression.
This study reveals that cohesin-mediated loop extrusion collisions, rather than CTCF occupancy alone, actively maintain ordered nucleosome architecture at CTCF sites and transcription start sites.
This study establishes a generalizable end-to-end workflow for de novo peptide drug discovery by using deep learning to design, optimize, and validate a novel cyclic MC4R agonist that achieves high potency and significantly reduces food intake in mice.
Through high-throughput mutagenesis and SHAPE-MAP analysis of the MAP3K7 intron, this study reveals that while branchpoint mutations are the primary drivers of cryptic splice-site activation in the context of SF3B1 mutations, accurate splicing is maintained by extensive RNA structural variability rather than a single conserved structure, except within specific RNA-binding protein hotspots where structural similarity correlates with splicing outcomes.
The paper introduces Easymode, a library of pretrained general segmentation networks trained on over 4,000 tilt series that enables flexible, training-free workflows for identifying, extracting, and analyzing macromolecules in cellular cryo-ET, demonstrated by determining the 4.0 Å in situ structure of IMPDH filaments and mapping their cellular environment.
This study demonstrates that low-dose radiation-induced adaptive response in human lymphocytes significantly reduces initial DNA double-strand break signaling (measured by γH2AX foci) in a time-dependent and cell-type-specific manner, with maximal protection occurring at 15 hours post-priming.
This paper presents a generalized microfluidic strategy that uses affinity tags to capture and photo-elute proteins directly from minimal lysate or in vitro translation volumes, enabling high-resolution Cryo-EM structure determination while drastically reducing sample consumption and preparation time compared to conventional workflows.
This study presents a large-scale structural proteomics resource for *Arabidopsis thaliana* generated via an optimized PhoX cross-linking mass spectrometry workflow, which identified over 52,000 cross-linked peptide pairs defining thousands of protein-protein interactions and providing residue-level spatial constraints for diverse molecular machines such as photosystems, ribosomes, and histone complexes.
This study demonstrates that in murine lupus, thoracic aortic perivascular adipose tissue undergoes a coordinated molecular reprogramming characterized by interferon-driven inflammation, mitochondrial bioenergetic failure, and acquired impairment of adipogenic progenitor differentiation, collectively driving lupus-associated vasculopathy.